Threats of forest degradation of the Tano Offin Globally Significant Biodiversity Area present the need to generate eco-information pertinent for its conservational purposes. Ten 50m x 50m plots (tree layer) were assessed for plant life forms with diameter >= 10 cm. A 10m x 10m plot (shrub layer) was located within each of the 50m x 50m plots where plant life forms with diameter < 10 cm were assessed. 1m x 1m quadrats (herb layer) were laid at the corners of the 50m x 50m plots and its centre for canopy closure and natural regeneration assessments. Plant species (240) belonging to 59 families were identified: 171 trees, 41 lianas, 11 shrubs, 7 herbs, 7 herbaceous climbers, 1 epiphyte, 1 grass and 1 fern. Species diversity (H') of the tree, shrub and herb layers was 2.55, 2.54 and 2.48 respectively. The average maximum tree height was 46.19m and the basal area was 28.36 m(2)/ha, which is below the 35 m(2)/ha conventional basal area value of tropical forests. Celtis mildbraedii and Rinorea welwitschii were the most structurally significant species at the tree and shrub layers, respectively, and a total of 75 tree species were regenerating.