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Characterization of new microsatellite loci from Vitis vinifera and their conservation in some Vitis species and hybrids

Journal Article

Lefort F; Kyvelos C; Zervou M; Edwards K; Roubelakis-Angelakis K

2002

Molecular Ecology

2

20-21

We present here characterization data for seven new microsatellite markers designed from new microsatellite loci isolated from a microsatellite-enriched DNA library from Vitis vinifera. The observed heterozygosity varied from 0.73 up to 0.93 and the number of alleles per locus ranged from 12 to 26. This high polymorphism makes these new markers interesting for use in genotyping studies and completing the set of microsatellite markers already available for V. vinifera. Additionally these seven new markers appear to be conserved in four other Vitis species and 15 Vitis hybrids used as rootstocks for V. vinifera cultivation.

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The Liana Ecology Project is supported by Marquette University and funded in part by the National Science Foundation.